Effect of VEGF overexpression on the Bcl-2 of TAMs/MCF-7 cell co-culture system
10.3969/j.issn.1009-9905.2017.12.001
- VernacularTitle:乳腺癌TAMs/MCF-7细胞共培养体系中VEGF过表达对Bcl-2的影响
- Author:
Wei LIU
1
;
Jie AN
;
Hui-Chi HOU
;
Kang LI
;
Zhi LI
;
Si-Hu ZHEN
;
Shao-Wen WANG
Author Information
1. 中国人民解放军白求恩国际和平医院普外一科 河北石家庄050082
- Keywords:
Breast neoplasmas;
Tumor-associated macrophages;
Bcl-2;
Vascular endothelial growth factor
- From:
Chinese Journal of Current Advances in General Surgery
2017;20(12):925-928
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of VEGF overexpression on the Bcl-2 of TAMs /MCF-7 cell co-culture system.Methods:Application of PMA and IL-4 cells induced THP-1 cell differentiation into TAMs in vitro;TAMs and MCF-7 cell were co-cultured in non-contact Transwell system.MCF-7 cells' proliferation status after co-culture were detected by MTT method;Effect of VEGF over-expression in co-cultured system on Bcl-2 level s in the two cell lines by Western blot assay.Results:PMA and IL-4 induced THP-1 cell become TAMs in vitro.After co-cultured with TAMs 24 h,48 h,MCF-7 cell's proliferation activity increased by 16.16 ％ and 33.99％ vs the control group respectively.TAMs,MCF-7 cells were added VEGF and the supematant of co-culture system respectively,then Bcl-2 levels in both cells were significantly higher,the difference was statistically significant (P＜0.05).Conclusions Tumors secrete VEGF and other chemokines to recruit and activate TAMs.Proliferation,apoptosis and progression of tumor was affected by VEGF/Bcl-2 paracrine loop in tumor microenvironment.
