Inhibitory effects of tamoxifen on thyroid papillary carcinoma
10.3969/j.issn.1009-9905.2017.11.004
- VernacularTitle:他莫西芬对甲状腺乳头状癌的抑制作用
- Author:
Geng-Yu CHEN
1
;
Shu-Min LIU
;
Feng-Jiao LI
;
Huan-Yu LU
;
Xing-Song TIAN
Author Information
1. 山东大学附属省立医院乳腺甲状腺外科
- Keywords:
Tamoxifen;
Thyroid papillary carcinoma;
Cell proliferation;
Cell migration;
Cell invasion
- From:
Chinese Journal of Current Advances in General Surgery
2017;20(11):856-860
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of tamoxifen on proliferation,migration and invasion of human thyroid papillary carcinoma BCPAP cells.Methods:Human thyroid papillary carcinoma BCPAP cells were treated with various concentrations of tamoxifen for 24 hours,respectively,CCK8 assay was used to detect the change of proliferation level;It can be divided into four groups:negative group,10 μmol/L TAM group and 20 μmol/L TAM group.Wound healing test was used to detect the change of migration ability and Transwell assay was used to detect the change of invasion ability.The protein levels of bcl-2 and C-myc in the BCPAP cells treated with various concentrations of tamoxifen were detected by Western blot.Results:CCK8 assay,wound healing test and Transwell assay showed that tamoxifen had induced a dose-dependent decrease in the proliferation,migration and invasion of human thyroid papillary carcinoma BCPAP cells.Furthermore,western blot demonstrated that the tamoxifen decreased the expression of bcl-2 and C-myc in BAPAP cells.Conclusions Tamoxifen can inhibit proliferation,migration and invasion of human thyroid papillary carcinoma BCPAP cells.We speculated that the inhibition of proliferation maybe related to down-regulation of bcl-2 and C-myc expression.
