The MAK16 Gene of Entamoeba histolytica and Its Identification in Isolates from Patients.
10.3347/kjp.2014.52.4.429
- Author:
Maria del Pilar CRISOSTOMO-VAZQUEZ
1
;
Victor Alberto MAREVELEZ-ACOSTA
;
Andres FLORES-LUNA
;
Enedina JIMENEZ-CARDOSO
Author Information
1. Hospital Infantil de Mexico Federico Gomez, Laboratory of Parasitology Research, Dr. Marquez #162 Col. Doctores, 06720, Mexico, D.F. enedina@servidor.unam.mx
- Publication Type:Brief Communication
- Keywords:
Entamoeba histolytica;
subtraction hybridization;
mak16 gene;
amebic liver abscess
- MeSH:
Adolescent;
Animals;
Cricetinae;
Entamoeba histolytica/*genetics;
Gene Expression;
*Genes, Protozoan;
Genetic Association Studies;
Humans;
Liver Abscess, Amebic/*genetics/*parasitology;
Male;
Virulence Factors/*genetics;
Young Adult
- From:The Korean Journal of Parasitology
2014;52(4):429-433
- CountryRepublic of Korea
- Language:English
-
Abstract:
To identify sequences of Entamoeba histolytica associated with the development of amebic liver abscess (ALA) in hamsters, subtractive hybridization of cDNA from E. histolytica HM-1:IMSS under 2 growth conditions was performed: 1) cultured in axenic medium and 2) isolated from experimental ALA in hamsters. For this procedure, 6 sequences were obtained. Of these sequences, the mak16 gene was selected for amplification in 29 cultures of E. histolytica isolated from the feces of 10 patients with intestinal symptoms and 19 asymptomatic patients. Only 5 of the 10 isolates obtained from symptomatic patients developed ALA and amplified the mak16 gene, whereas the 19 isolates from asymptomatic patients did not amplify the mak16 gene nor did they develop ALA. Based on the results of Fisher's exact test (P<0.001), an association was inferred between the presence of the mak16 gene of E. histolytica and the ability to develop ALA in hamsters and with the patient's symptoms (P=0.02). The amplification of the mak16 gene suggests that it is an important gene in E. histolytica because it was present in the isolates from hamsters that developed liver damage.
