Microsatellite marker design and genetic analysis for the common marmoset
10.3969/j.issn.1671-7856.2018.06.004
- VernacularTitle:实验用狨猴微卫星引物筛选及遗传多样性分析
- Author:
Yongkang TENG
1
;
Xianju LIU
;
Xu ZHANG
;
Zhiguang XIANG
;
Yanshuo RUAN
;
Chong XIAO
;
Yunbo LIU
Author Information
1. 中国医学科学院医学实验动物研究所
- Keywords:
marmoset;
microsatellite DNA;
genetic diversity
- From:
Chinese Journal of Comparative Medicine
2018;28(6):15-20,28
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen and optimize the microsatellite DNA primers of the laboratory common marmoset, analyze and evaluate the population genetic quality for the marmosets (Callithrix jacchus) introduced into the Institute of Medical Laboratory Animal science, Chinese Academy of Medical Sciences. Methods A total of 30 marmosets were randomly chosen, and their genome DNA from blood was extracted using phenol/chloroform method. The microsatellite DNA was amplified using standard polymerase chain reaction (PCR). The amplification products were tested by STR scanning after 2% agrose gel and 8% PAGE electrophoresis. The data processing and genetic analysis were completed using the Popgene1. 32 software. Results A total of 20 pairs of microsatellite loci showed genetic polymorphism, and 147 alleles were detected. The number of allele was 5 to 10, average 7. 35. The effective allele was 2. 2500 to 6. 3830, average 4. 0402. The observed heterozygosity was 0. 000 to 0. 4667, average 0. 1533. The expected heterozygosity was 0. 1424 to 0. 4350, average 0. 2506. The Shannon diversity index was 1. 2242 to 2. 0324, average 1. 5949. The polymorphic information content was 0. 5366 to 0. 8254, average 0. 7053. Conclusions The 20 pairs of marmoset microsatellite primers are genetically highly diverse and are in a Hardy-Weinberg equilibrium.
