Botulinum neurotoxin serotype A heavy chain intervenes in the H3 acetylation: A preliminary study
10.3969/j.issn.1671-7856.2018.06.002
- VernacularTitle:A型肉毒素重链干预神经细胞组蛋白3乙酰化水平的初步研究
- Author:
Jing LAN
1
;
Fu LIU
;
Yang GAO
;
Zhiqiang LI
;
Ya LIU
;
Mingwei ZHAO
;
Haixia YUAN
;
Xiaqing LI
Author Information
1. 山西医科大学病理生理教研室
- Keywords:
botulinum neurotoxin serotype A,BoNT/A;
BoNT/A heavy chain;
histone acetylation;
acetylation/deacetylation;
neuritogenesis;
spinal cord injury,SCI
- From:
Chinese Journal of Comparative Medicine
2018;28(6):4-9
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and molecular mechanism of botulinum neurotoxin serotype A (BoNT/A) heavy chain on neuron regeneration. Methods Cell culture, rats, immunofluorescence, SDS-PAGE and western blot, etc. were adopted in this study to explore the alterations of histone-3 acetylation (acetyl-H3 ) by local treatment of BoNT/A heavy chain to spinal cord injury (SCI) in rats (in vivo) or by adding it into cell culture (in vitro). Meanwhile, the relevance of acetyl-H3 to neurite out-growth based on SCI and cell culture with BoNT/A heavy chain application was approached as well. Results The application of BoNT/A heavy chain to cultured Neuro-2a cells increased the level of H3 acetylation. The increase of H3 acetylation was paralleled with the growth of neuritogenesis. Also, the neuronal treatment of BoNT/A heavy chain to SCI promoted the re-growth of neuronal processes surrounding the lesions. The growth of neuronal processes was positively correlated to the level of H3 acetylation. During the periods of BoNT/A heavy chain treatment in vivo or in vitro, the increase of H3 acetylation showed two peaks. Conclusions BoNT/A heavy chain increased the H3 acetylation, which might be one of its neuritogenic mechanisms.
