In vitro method for skin sensitization based on dendritic cell activation

VernacularTitle:基于树突状细胞激活建立皮肤致敏的体外方法
Author: Jian CHEN ¹ ; Yihui KE ; Yu CHEN ; Weijun TAN ; Shujun CHENG
Author Information

1. 广州市华代生物科技有限公司
Keywords: skin sensitization; dendritic cells; DCs; alternative methods; adverse outcome pathway; AOP
From: Chinese Journal of Comparative Medicine 2018;28(5):114-118
CountryChina
Language:Chinese
Abstract: Allergy contact dermatitis is a type IV delayed type hypersensitivity that is induced by exogenous compounds and involves many cell types. Traditional animal testing uses guinea pigs or mice as a model. With progress of the adverse outcome pathway(AOP)on skin sensitization, a concept for development of alternative methods based on a molecular initiating event and key events is provided. Dendritic cell(DC)activation plays a key role in the AOP. Many alternative methods have been developed,with several methods validated and accepted as guidance for assays. This paper examines DC screening, characteristics of test parameters, and limitations and applicability of DC-derived methods. Progress on interactions between DCs and other cells, co-culture systems, and the human body-on-a-chip will also be introduced. Altogether,this paper will provide information for optimization of in vitro alternative methods for sensitization detection.