An improved method of collecting cerebrospinal fluid in SD rats

VernacularTitle:SD大鼠脑脊液采集方法的改进
Author: Yixin ZHU ¹ ; Dongqing YE ; Xiaoli LI
Author Information

1. 东南大学附属中大医院神经内科
Keywords: rats; cerebrospinal fluid; collection method
From: Chinese Journal of Comparative Medicine 2018;28(4):113-115
CountryChina
Language:Chinese
Abstract: Objective To explore a rapid, convenient and non-polluting method of collecting cerebrospinal fluid (CSF)in Sprague Dawley(SD)rats for beginners. Methods Forty-five SD rats were divided into three groups—group 1(collecting CSF through neck muscles), group 2(collecting CSF through skin directly), and group 3(collecting CSF through endorhachis). Results The successful rate of the CSF collection in the group 1 was 86.7%, group 2 was 46.7%,and group 3 is 33.3%. Conclusions The modified collection method of CSF by cisterna puncture through neck muscles in SD rats is simple,convenient,efficient and non-polluting,and is especially suitable for beginners.