Protective effect of diallyl disulfide against the radiation damage in germ cells of male mice
10.3969/j.issn.1671-7856.2018.04.005
- VernacularTitle:二烯丙基二硫对小鼠生精细胞的辐射防护作用
- Author:
Shuai XU
1
;
Xixia LI
;
Min WANG
;
Shuping LI
;
Weixiong LIAO
Author Information
1. 肇庆医学高等专科学校
- Keywords:
diallyl disulfide;
X-ray;
mouse;
spermatzoon;
radioprotection
- From:
Chinese Journal of Comparative Medicine
2018;28(4):27-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the radiation-protective effect of diallyl disulfide(DADS)in germ cells of male mice. Methods Male mice were whole-body exposured to 4 Gy X-ray irradiation to establish a animal radiation damage model. Testicular histology, sperm motility and sperm deformity rate were observed, protein carbonyl content, malondialdehyde(MDA)and 8-hydroxy deoxyguanosine(8-OHdG)content were tested to assess the degrees of radiation damages of sperm cells and protective effect of DADS. Testicular tissue antioxidant enzymes such as superoxide dismutase (SOD),catalase(CAT),glutathione peroxidase(GSH-px)activity and glutathione(GSH)content were examined. The expression of Nrf2 signal protein was detected to explore the radiation-protective mechanism of DADS. Results Compared with the pure exposure group, the testicular tissue damages in the DADS pretreatment group were milder, sperm motility increased significantly(P< 0.05)and sperm deformity rate decreased(P< 0.05), and the protein carbonyl content, MDA and 8-OHdG levels significantly reduced(P< 0.05). The antioxidant indexes of SOD, CAT, GSH-px and GSH were markedly improved(P< 0.05),and the expression of Nrf2 was dramatically enhanced. Conclusions DADS has a protective effect on acute radiation injury in germ cells of male mice by means of anti-oxidative ability.
