Inhibitory effect of rosuvastatin on migration and invasion of U87 glioma cells in vitro

VernacularTitle:瑞舒伐他汀对胶质瘤U87细胞迁移、侵袭的抑制作用
Author: Ming LIU ¹ ; Xipeng LIU
Author Information

1. 河北北方学院附属第一医院神经外科
Keywords: rosuvastatin; glioma cells; migration; invasion
From: Chinese Journal of Comparative Medicine 2018;28(3):52-56
CountryChina
Language:Chinese
Abstract: Objective To investigate the inhibitory effect of rosuvastatin on the migration and invasion of U87 glioma cells and its related mechanisms. Methods Cultured U87 cells were treated with 0, 5, 10 and 20 μmol/L rosuvastatin for 24,48 and 72 h. Cell viability was measured by CCK-8 assay. Migration ability of the cells was detected by scratch assay,and invasion ability of the cells was detected by Transwell assay. The expression of matrix metalloproteinase 2(MMP2),MMP9 and the Wnt/β-catenin signaling pathway-related proteins was detected by Western blot assay. Results Compared with the control group,the viability of U87 cells in the 5,10 and 20 μmol/L rosuvastatin groups was decreased (P < 0.01)in a time- and dose-dependent manner. Migration and invasion abilities of the cells were decreased(P <0.01). In addition,the expression levels of MMP2,MMP9,Wnt1,Wnt3a,Wnt7a,and β-catenin were decreased as well (P < 0.01). Conclusions Rosuvastatin can significantly inhibit the migration and invasion abilities of U87 glioma cells, probably related to the blocking of Wnt/β-catenin signaling pathway.