Construction and characterization of a eukaryotic expression vector of full-length and mature IL-37b
10.3969.j.issn.1671-7856.2018.02.010
- VernacularTitle:全长与成熟形式IL-37 b的真核表达载体的构建与研究
- Author:
Mengyuan LI
1
;
Rongfei WEI
;
Xingjiu YANG
;
Ruimin ZHU
;
Ran GAO
Author Information
1. 中国医学科学院医学实验动物研究所
- Keywords:
IL-37b;
eukaryotic expression vector;
gene cloning
- From:
Chinese Journal of Comparative Medicine
2018;28(2):59-63
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a eukaryotic expression vector pEGFP N1/IL-37b of full-length and mature IL-37b,and to detect the expression of both full-length and mature IL-37b in RAW 264.7 cells, a mouse macrophage cell line. Methods To construct the eukaryotic vectors of full-length and mature IL-37b by using plasmid pUBC/IL-37b as a template containing the coding region of IL-37b full-length gene. To detect the expression of IL-37b by western blot and confocal microscopy after transfected the recombinant plasmid into RAW 264.7 cells, and to detect the inhibition of full-length and mature IL-37b on IL-6 production by real-time PCR. Results Eukaryotic vectors pEGFP N1/IL-37b expressed full-length and mature IL-37b after transfection in cells, which inhibited LPS-induced IL-6 production. Conclusions Eukaryotic vectors of full-length and mature IL-37b can be successfully constructed,and lays a foundation for further study of anti-inflammation functions and mechanisms of IL-37b.
