ism1 regulates convergence and extension movements during zebrafish gastrulation

VernacularTitle:isthmin 1对斑马鱼胚胎汇聚延伸运动的影响
Author: Zheng WANG ¹ ; Jinpeng LI ; Yifang YAN ; Qiang WANG
Author Information

1. 河北省安国中学
Keywords: Ism1; convergence and extension movements; gastrulation; zebrafish
From: Chinese Journal of Comparative Medicine 2018;28(2):40-45
CountryChina
Language:Chinese
Abstract: Objective During zebrafish embryo gastrulation, the embryonic body plan is established by coordinated movements,including epiboly,involution,and convergence and extension(CE)movements. The aim of this study was to examine the expression patterns and the developmental functions of isthmin 1(ism1)during zebrafish gastrulation. Methods First, the expression of ism1 was examined using whole mount in situ hybridization during zebrafish embryonic development. Next,antisense morpholino oligonucleotide against ism1 and ism1 mRNA were injected into zebrafish embryos,respectively,to investigate its role in CE movements. Results ism1 is an early zygotic gene that is expressed in the dorsal side at 30% epiboly stage and in the blastoderm margin with the highest level in the dorsal organizer at shield stage as revealed by whole-mount in situ hybridization experiments. Compared with the wild-type embryos,either gain-or loss-of-function of ism1 leads to severe defects of CE movements. Conclusions Ism1 plays an essential role in CE movements during zebrafish embryo gastrulation.