Feasibility study of acupuncture treatment on the miniature pig model of chronic myocardial ischemia
10.3969/j.issn.1671-7856.2018.01.019
- VernacularTitle:针刺治疗慢性心肌缺血小型猪模型系列方法的可行性探讨
- Author:
Jinling ZHANG
1
;
Yi TIAN
;
Peijing RONG
;
Jiliang FANG
;
Yu WANG
;
Li SHI
;
Shuyong JIA
;
Xun HE
;
Hongtao LEI
;
Hong MENG
Author Information
1. 中国中医科学院针灸研究所
- Keywords:
large laboratory animals;
Bama miniature pigs;
chronic myocardial ischemia;
needle-embedding electroacupuncture;
gross pathology;
electron microscopic ultrastructure
- From:
Chinese Journal of Comparative Medicine
2018;28(1):108-112
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of needle-embedding therapy in the treatment of chronic myocardial ischemia using a miniature pig model established by placement of an Ameroid constrictor at the left anterior descending branch (LAD) of coronary artery in Bama miniature pigs during surgery. Methods The miniature pig model of chronic myocardial ischemia was established by placement of an Ameroid constrictor at the left anterior descending branch of the left coronary artery in Bama miniature pigs. The pig models were randomly divided into the treatment group (the"Neiguan " group) and the control group (the "Zusanli " group), and were treated with needle- embedding electroacupuncture at the"Neiguan" (PC6) and "Zusanli" (ST36) acupoints, respectively. Myocardial samples were taken at 6 weeks after surgery for light and electron microscopic examinations. Results Gross pathology showed that ischemic area in the myocardium appeared in both experimental groups. The ischemic area in the "Zusanli "group was larger than that of the"Neiguan"group. Histopathology showed that the acupuncture treatment at the"Neiguan"acupoint reduced the ischemic injury in the pig myocardial tissues. Ultrastructural observation of the myocardium showed mitochondrial vacuolization in cardiomyocytes and myocardial fibrosis in both groups. Conclusions Acupuncture therapy at the"Neiguan"acupoint of pericardial channel may exert protective effect on the myocardial ischemia by reducing the ischemia-injury of cardiomyocytes, but can not inhibit the already existed ischemia-induced cardiomyocytic injuries. Our findings suggest that the establishment of miniature pig model of chronic myocardial ischemia by surgically placing an Ameroid constrictor on the left anterior descending branch of left coronary artery and the needle-embedding in acupoints is feasible for the treatment of chronic myocardial ischemia in this pig model.
