Characteristics and changes of the CD molecules and IFN-γ expressions in tree shrews infected with mammalian orthoreovirus
10.3969/j.issn.1005-4847.2018.03.009
- VernacularTitle:呼肠孤病毒感染的树鼩中三类CD分子及IFN-γ表达变化及特征
- Author:
Yuan YUAN
1
;
Xuan WANG
;
Zhicheng ZHANG
;
Na LI
;
Wenguang WANG
;
Dexuan KUANG
;
Jiejie DAI
Author Information
1. 中国医学科学院/北京协和医学院医学生物学研究所
- Keywords:
orthoreovirus of tree shrew;
immune response;
flow cytometry;
cluster of differentiation (CD);
cytokine
- From:
Acta Laboratorium Animalis Scientia Sinica
2018;26(3):323-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the characteristics of immunological changes in tree shrews infected with orthoreovirus, and provide a theoretical basis for the prevention of virus in tree shrews. Methods 40 -50-day-old tree shrews were divided into three groups: MRV1/TS/2011 virus-infected and MRV3/TS/2013 virus-infected groups, and saline-treated control group. On the 1, 8, 14, 21, and 28 days after infection, blood samples were taken from the tail vein and used for RT-PCR, flow cytometry and ELISA detection, to assess the viral load, number of CD4/CD8/CD19 cells, and IFN-gamma expression. Results The MRV1/TS/2011 and MRV3/TS/2013 viral load in the plasma and the number of CD4 +and CD19 +cells reached a peak at the 14th day after infection. At the first day after MRV1/TS/2011 infection, the CD4 +cells had a significantly higher expression compared with the normal group. CD8 +cells and the IFN-gamma expression reached a peak at the 21st day after infection. The expression of CD4 +was even higher after MRV1/TS/2011 infection, and the expression of CD8 +cells was higher after MRV3/TS/2013 infection. Conclusions We would conclude that after MRV1/TS/2011 and MRV3/TS/2011 virus infection, accompanying the changes of viral load, it shows some regularity of the expression of CD4/CD8/CD19 and IFN-gamma in the tree shrews: at the early stage of MRV1/TS/2011 virus infection, humoral immunity is stimulated, and CD4 +cells play a major role. MRV3/TS/2013 virus may mainly affect the cellular immunity, while humoral immunity only plays a role at a high viral expression or the late stage of infection. CD4 +cells may be more sensitive to type 1 reovirus, and CD19 +cells may be more sensitive to type 3 reovirus.
