Establishment and characterization of a patient-derived orthotopic xenograft ( PDOX) model of pancreatic cancer
10.3969/j.issn.1005-4847.2018.03.005
- VernacularTitle:基于临床手术标本的胰腺癌原位移植模型建立及评价
- Author:
He ZHANG
1
;
Caiqin ZHANG
;
Yong ZHAO
;
Dengxu TAN
;
Changhong SHI
Author Information
1. 沈阳军区总医院医务部
- Keywords:
pancreatic cancer;
patient-derived orthotopic xenograft(PDOX) model;
near infrared fluorescence (NIRF);
optical imaging;
positron emission tomography(PET)
- From:
Acta Laboratorium Animalis Scientia Sinica
2018;26(3):296-301
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and evaluate a patient-derived orthotopic xenograft ( PDOX ) model of pancreatic cancer. Methods Tissues of patient-derived pancreatic tumor were transplanted into nude mouse pancreas by surgery. The PDOX models were evaluated by the small animal near infrared fluorescence ( NIRF) optical imaging and PET/CT. The traceability of PDOX models was detected by STR technology, and the pathological changes were observed by H&E staining, immunohistochemistry, and serum level of CA19-9 was detected by ELISA. Results Apparent NIRF were observed to be accumulated in pancreatic site by optical imaging system. The location and size of the xenografts tumor were revealed by fluorescence intensity. The PET/CT images with 18F-FDG molecular probe confirmed the tumor's location and size. Ex vivo NIRF imaging of isolated organ further showed the tumor formation. The traceability of PDOX models was 99. 99% with human origin. H&E staining pathology and immunohistochemistry indicated the pancreatic cancer characteristics. The high serum level of ca19-9 confirmed the mice bearing tumor. Conclusions Pancreatic PDOX models are successfully established in this study, and it can be evaluated comprehensively by NIRF optical imaging and PET/CT, providing an appropriate platform for further research of pancreatic cancer.
