Role of 1α,25(OH)2D3in the metabolism of aggrecan and aggrecanase in chondrocytes of rat articular cartilage
10.3969/j.issn.1005-4847.2018.03.004
- VernacularTitle:1α,25(OH)2D3对大鼠关节软骨细胞蛋白聚糖和蛋白聚糖酶代谢的调节作用
- Author:
Jian GUAN
1
;
Qizhao TAN
;
Zhenda ZHAO
;
Zhongjun LIU
;
Chunli SONG
;
Huijie LENG
Author Information
1. 北京大学第三医院骨科
- Keywords:
1α;
25(OH)2D3;
aggrecan;
aggrecanase;
chondrocytes;
osteoarthritis
- From:
Acta Laboratorium Animalis Scientia Sinica
2018;26(3):287-295
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of vitamin D in the synthesis and degradation of aggrecan in rat articular chondrocytes at cellular level. Methods Rat articular chondrocytes were stimulated by IL-1α, IL-1β and TNF-α, respectively. Normal and inflammatory chondrocytes were treated with different doses of vitamin D, respectively. CCK8, Flow cytometry, real time-PCR and western blot analysis were used to examine the proliferation activity and apoptosis level of chondrocytes, and the expression of aggrecan, ADAMTS-4 and ADAMTS-5 at both mRNA and protein levels. Results IL-1α,IL-1β and TNF-α significantly decreased the proliferation activity and increased the apoptosis level of the chondrocytes. Furthermore, IL-1α, IL-1β and TNF-α significantly decreased the expression of aggrecan, and increased the expressions of ADAMTS-4 and ADAMTS-5 at both mRNA and protein levels in the chondrocytes. 1α,25 (OH)2D3supplementation significantly increased the proliferation activity and decreased the apoptosis level of chondrocytes stimulated by IL-1α, IL-1β and TNF-α in a dose-dependent manner, but not affected the normal chondrocytes. Meanwhile, 1α,25(OH)2D3also significantly increased the expression of aggrecan, and decreased the expressions of ADAMTS-4 and ADAMTS-5 at both mRNA and protein levels in the chondrocytes under inflammatory conditions. Conclusions Vitamin D may promote the anabolism of aggrecan and inhibit aggrecanase activity in chondrocytes under inflammatory conditions, which may impact overall protection for articular cartilage.
