Correlation between gut microbiota and anti-dsDNA antibody in the mouse model of systemic lupus erythematosus
10.3969/j.issn.1005-4847.2018.02.009
- VernacularTitle:系统性红斑狼疮小鼠肠道微生物与抗dsDNA抗体水平的相关性
- Author:
Guixiang ZOU
1
;
Xinwang DUAN
;
Haitao NIU
Author Information
1. 南昌大学第二附属医院 风湿免疫科
- Keywords:
gut microbiota;
systemic lupus erythematosus;
Anti-dsDNA antibody;
mice
- From:
Acta Laboratorium Animalis Scientia Sinica
2018;26(2):195-200
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between gut microbiota and anti-dsDNA antibody in systemic lupus erythematosus(lupus)TC mice. Methods ELISA was performed to detect serum anti-dsDNA antibody in the lupus TC mice. Then,the feces of both dsDNA-positive and -negative mice were collected, and 16S rRNA of the stool sample was sequenced by Illumina HiSeq 2500 high-throughput sequencing,to analyze the relationship between gut microbiota and anti-dsDNA antibody level in the two groups. Results The result of ELISA and mouse fecal high-throughput sequencing showed that the species diversity of gut microbiota in the dsDNA-positive TC mice was significantly lower than that in the dsDNA-negative TC mice. The gut microbiota of TC mice in the two groups showed significant differences at different classification levels: Chloroflexi at phylum level, Betaproteobacteria, Deltaproteobacteria and Ktedonobacteria at class level,Burkholderiales,Desulfovibrionales and Ktedonobacterales at order level,Alcaligenaceae and Desulfovibrionaceae at family level,and Parasutterella and Desulfovibrio at genus level(P<0.05 for all). Conclusions The flora composition of gut microbiota in lupus TC mice is highly correlated with anti-dsDNA antibody. The results of our study provide a basis for further elucidation of the relationship between gut microbiota and the pathogenetic mechanism of systemic lupus erythematosus.
