Comparative analysis of intestinal flora of Microtus fortis living under laboratory feeding and wild survival conditions
10.3969/j.issn.1005-4847.2018.02.008
- VernacularTitle:实验室饲养和野外生存条件下东方田鼠肠道菌群的多样性
- Author:
Jie FENG
1
;
Zhimin SHEN
;
Shengchang WANG
;
Jinxing LIN
;
Xiong BO
;
Jianyun XIE
Author Information
1. 上海实验动物研究中心
- Keywords:
Microtus fortis;
intestinal flora;
16S rDNA;
bacterial diversity
- From:
Acta Laboratorium Animalis Scientia Sinica
2018;26(2):188-194
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the differences of bacterial distribution of intestinal flora in Microtus fortis living under laboratory feeding and wild survival conditions. Methods The 16S rDNA-V4-V5 region of bacteria in the ileocecal contents from Microtus fortis raised in lab and captured in wild were measured by high-throughput sequencing. The number of operational taxonomic units(OTUs)were sorted and calculated,and the species abundance and distribution and difference were analyzed. Results The rarefaction curves indicated that adequate sampling was achieved. At the phylum level,the distribution of intestinal flora between two groups was similar. The experimental group had a unique phylum, Lentisphaerae. The wild type group had 3 unique phylums,Fusobacteria,Thaumarchaeota and an unclassified phylum. At the genus level, the kind of intestinal flora in the wild type group was more abundant than the experimental group. Ruminococcus is the largest differential genus. Conclusions The microbial community structure and differences of Microtus fortis living under different conditions are obtained. It may further enrich the basic biology data of Microtus fortis.
