Effect of leptin on expression of lipoic acid synthase in the liver and kidney of Leprdb/dbmice
10.3969/j.issn.1005-4847.2018.02.002
- VernacularTitle:硫辛酸合成酶在Leprdb/db小鼠肝肾中的表达
- Author:
Qiang PENG
1
;
Yingzheng ZHAO
;
Tingting YAN
;
Xiaonan ZHAI
;
Xuxu ZHANG
;
Xianwen YI
;
Hexi ZHANG
;
Guangcui XU
Author Information
1. 新乡医学院公共卫生学院
- Keywords:
Leprdb/dbmice;
lipoic acid;
liver;
kidney;
leptin
- From:
Acta Laboratorium Animalis Scientia Sinica
2018;26(2):145-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of lipoic acid synthase(LIAS)in the liver and kidney of Leprdb/db mice with deficient leptin receptor. Methods Eight 10-week old male Leprdb/ +mice and Leprdb/dbmice were included in this study. The body weight of rats in the two groups was measured. Fasting blood glucose(FPG)was measured with blood glucose test strips for all mice after fasting for 8 hours. Blood samples were obtained from the abdominal aorta and the animals were sacrificed. The liver and kidney were weighed. The right lobe of liver and the left kidney samples were fixed in 4% paraformaldehyde for pathological examination. Serum samples were separated and the sereum contents of CHO, TG,HDL and LDL were detected. The mitochondria of liver and kidney tissues were extracted with a mitochondrial isolation kit, and the protein was extracted. The expression of LIAS protein was detected by western blot. Results Histopathological observation showed that the liver and kidney tissues of Leprdb/ +mice have intact and clear structure. But the liver tissue of Leprdb/dbmice showed fatty degeneration, the kidney tissue showed glomerular hypertrophy, basement membrane thickening, mesangial area widened, including mesangial cells and mesangial matrix increased. The GLU,CHO,TG,LDL and AST of Leprdb/dbmice were significantly increased compared with those of Leprdb/ +mice(P<0.05). Compared with Leprdb/ +mice,the LIAS protein expression was significantly increased in the liver and kidney mitochondria of Leprdb/dbmice(P<0.05). Conclusions There is impaired glucose and lipid metabolism in the Leprdb/dbmice which has defect leptin receptor,and the expression of LIAS protein in liver and kidney of the Leprdb/dbmice is higher than that of Leprdb/ +mice.
