Establishment of a rat model of lumbar spinal root avulsion
10.3969/j.issn.1005-4847.2018.01.016
- VernacularTitle:大鼠腰椎腹侧神经根牵拉模型的建立
- Author:
Jihong LIU
1
;
Lijun GE
;
Yan CHEN
;
Yong ZHANG
;
Jiaqi LU
;
Ailian LIU
;
Ning LI
;
Huaichang SUN
;
Jianfei WANG
Author Information
1. 扬州大学兽医学院
- Keywords:
spinal root avulsion;
brain-derived neurotrophic factor;
BDNF;
amyotrophic lateral sclerosis;
rats
- From:
Acta Laboratorium Animalis Scientia Sinica
2018;26(1):101-106
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rat model of spinal root avulsion and to validate the model by brain-derived neurotrophic factor(BDNF)treatment. Methods To evaluate the motor neuron loss,5 male SD rats were used to undergo spinal root avulsion surgery. One week later, the number of motor neurons in the ventral horn of the spinal cord was as-sessed by histopathology using immunohistochemical staining with a choline acetyl transferase(ChAT)antibody. After this pilot study,40 male SD rats at 7 weeks of age were randomly divided into 4 groups:two control groups,BDNF preventive and treatment groups. Results All rats recovered well post-surgery and no obvious abnormality was observed. Compared with the contralateral side,the number of motor neurons in the ipsilateral avulsed side was significantly decreased at one week after surgery(20.06%,P<0.05). Compared with the control group,there was a significant increase in ChAT posi-tive neurons in the BDNF preventive group(17.85% vs. 93.06%,P<0.0001)or BDNF treatment group(1week after surgery)(26.94% vs. 86.87%, P<0.0001), indicating that the motor neurons were effectively protected by BDNF. Conclusions A rat model of spinal root avulsion is successfully established,which can be valuable for studies of amyotro-phic lateral sclerosis and drug discovery efforts.
