Establishment of a rat xenograft model of human uterine leiomyoma
10.3969/j.issn.1005-4847.2018.01.014
- VernacularTitle:大鼠荷子宫肌瘤组织移植瘤模型的建立
- Author:
Yang SONG
1
;
Yuhua ZHEN
;
Yongge GUAN
;
Kunyin LI
;
Yudan GUO
Author Information
1. 广州中医药大学
- Keywords:
uterine leiomyoma;
xenografts;
wistar rats;
model;
immunosuppresive agent;
mycophenolate mofetil
- From:
Acta Laboratorium Animalis Scientia Sinica
2018;26(1):91-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rat xenograft model of human uterine leiomyoma using immunosuppressive a-gent and provide a useful tool for the study on uterine leiomyoma. Methods Intragastric administration with immunosup-pressive agent mycophenolate mofetil(MMF)(40 mg/kg)was given to rats for two weeks before the surgery until the end of the experiment. 20 SPF female Wistar rats were randomly divided into 4 groups after abdominal transplantation of human leiomyoma tissues:group A received femoston containing 0.4 mg/kg estradiol and 2 mg/kg dydrogesterone, group B re-ceived estadiol 0.4 mg/kg,group C received dydrogesterone 2 mg/kg,and group D served as the control group, received distilled water 1 mL/200 g. All rat received the corresponding drugs once per day for 2 days. Samples were taken at 4 weeks after the surgery to observe the pathology of the tumor tissues. Results The modeling success rates were 90% in the group A,40 % in the group B,and 0% in the groups C and D. Conclusions Rat xenograft model of human leiomyoma can be successfully established using an immunosuppressive agent femostone with a high modeling success rate and low cost. It can be used as a new animal model for the study of transplanted leiomyoma.
