Comparision on the effect of RT-LAMP and LAMP method for detection of Mycobacterium tuberculosis
10.3969/j.issn.1002-2694.2018.00.028
- VernacularTitle:RT-LAMP与LAMP法检测结核分枝杆菌的效果比较
- Author:
Dan-Dan WU
1
;
Bao-Sheng LI
;
Ji-Wen KANG
;
Dian-Xing SUN
Author Information
1. 承德医学院
- Keywords:
Mycobacterium tuberculosis;
reverse transcription loop-mediated isothermal amplification (RT-LAMP);
loop mediated isothermal amplification (LAMP);
16S rRNA
- From:
Chinese Journal of Zoonoses
2018;34(3):248-254
- CountryChina
- Language:Chinese
-
Abstract:
We compared the RT-LAMP,LAMP and L-J for detecting MTB to provide the rapid diagnosis method for tu-berculosis.In this assay,NTM and other common respiratory bacteria were used to detect specificity,Mycobacterium tubercu-losis specific products were identified by restriction enzyme digestion.To detect sensitivity,we used RT-LAMP,LAMP and L-J to detect 100 cases sputum specimens from patients with tuberculosis and 22 cases control sputum specimens,the 10 times dilution of 1 ng/μL H37Rv standard strains was used to detect RT-LAMP limit.The results showed that the positive rate of RT-LAMP,LAMP and L-J were 100%,92%,88%.RT-LAMP and L-J,RT-LAMP and LAMP were statistically signifi-cant,RT-LAMP had 10 times higher sensitivity than LAMP,RT-LAMP not only to identify viable but also capable of detec-ting a single copy of MTB.So RT-LAMP is superior to LAMP and L-J and is practical for use in primary medical care institu-tion or peripheral laboratory.
