Contamination and genotyping of Clostridium perfringens from chicken in Macao
10.3969/j.issn.1002-2694.2018.00.038
- VernacularTitle:澳门地区鸡源产气荚膜梭菌的污染情况及基因分型
- Author:
Zhong-Hao LU
1
;
Hui-Yi HUANG
;
Xiao-Jun LIANG
;
Zhi-Jun WU
;
Wan-Fen LI
Author Information
1. 澳门理工学院高等卫生学校
- Keywords:
Clostridium perfringens;
chicken;
multiplex PCR;
genotyping
- From:
Chinese Journal of Zoonoses
2018;34(3):236-238
- CountryChina
- Language:Chinese
-
Abstract:
We determine the contamination and genotypes of Clostridium perfringens isolated from chickens in Macau.C. perfringens were isolated from these samples by double tube method and then identified by biochemical test.Genotyping of i-solated strains was determined by multiplex PCR.A total of 120 samples,the positive rates were 37.5%,respectively.All of the isolated strains were type A.This study indicated that contamination of C.perfringens type A exists in chicken intestines, fresh chickens and chilled chickens in Macau,and provided reference data for the public health problems caused by C.perfrin-gens.