Hepatitis B spliced protein enhanced NF-κB activities via interaction with ubiquitously expressed transcript splice variant 1

VernacularTitle:乙型肝炎病毒剪接蛋白HBSP与泛转录表达因子剪接变异体1相互作用增强NF-κB活性
Author: Wan-Nan CHEN ¹ ; Yan-Fei YU ; Qiong WU ; Shen-Min LIU ; Long-Fei WU ; Jia-Jian SHI ; Xu LIN
Author Information

1. 福建医科大学基础医学院
Keywords: hepatitis B virus; RNA splicing; ubiquitously expressed transcript protein; nuclear factor kappa B
From: Chinese Journal of Zoonoses 2018;34(1):29-34,38
CountryChina
Language:Chinese
Abstract: We investigated whether hepatitis B spliced protein affect the NF-kappa B activities by interacting with ubiquitously expressed transcript splice variant 1 (UXT-V1).The HBSP-UXT-V1 protein interactions were screened by yeast two hybrid assay,and confirmed by immunoprecipitation,confocal microscopy,mammalian two hybrid assay,and GST-Pulldown assay.The reporter plasmids driven by NF-kappa B promoter were transfected into UXT-knockdown HBSP stably expressed cell lines,and the reporter genes were detected after transfection.Results showed that the interaction between UXT-V1 and HBSP in yeast was demonstrated.Furtherly,HBSP could interact with UXT-V1 in mammalian cells.HBSP could enhance NF-kappa B activities,and this effect was partly achieved by the interaction with UXT-V1.In conclusion,the effect of HBSPUXT-V1 interaction on the NF-kappa B pathway in hepatocytes may have an impact on HBV related liver diseases.