Prediction and analysis for secretory proteins from Thelazia callipaeda at Genome Scale
10.3969/j.issn.1002-2694.2017.00.003
- VernacularTitle:结膜吸吮线虫基因组中分泌蛋白的规模预测及分析
- Author:
Ming-Hui ZHENG
1
;
Chang-Lin YE
;
Lu-Fei ZHANG
;
Nan JIANG
;
Li-Fang HE
;
Hui LIU
Author Information
1. 遵义医学院
- Keywords:
Thelazia callipaeda;
secretome;
secretory protein;
sugar metabolism
- From:
Chinese Journal of Zoonoses
2018;34(1):12-17,22
- CountryChina
- Language:Chinese
-
Abstract:
We conducted prediction and analysis for secretory proteins from Thelazia callipaeda at Genome Scale based on the previous full genome annotation.The software SignalP,TMHMM,big-PI Predictor,MEME,Protcomp and SecretomeP were combined to process the prediction of the secretome of Thelazia callipaeda.The analyses of secretory proteins by GO function enrichment,KEGG pathway,and statistics of domains were performed.Results showed that totally 259 secretory proteins were found in Thelazia callipaeda genome and the amino acid lengths of secretory proteins were mainly concentrated between 100 to 700 aa exclusively.GO function analysis of secretory proteins indicated that they were enriched in the secreting pathways and in the interactions with host.The results of KEGG metabolism secretory proteins suggested that some of them contributed to drug metabolism and glutathione metabolism.And domain analysis suggested that most of them were glycoside hydrolase,contributing to sugar metabolism.Around 126 secretory proteins had antigenicity of B-cell epitope.In summary,we found that secretory proteins in Thelazia callipaeda were most small proteins,which were involved in sugar metabolism and antioxidative activity,facilitating Thelazia callipaeda to invade the hosts and play a key role in the parasitic course.
