Evolution of influenza A(H3N2) virus hemagglutinin (HA1) gene in Shangluo, China, 2014-2015
10.3969/j.issn.1002-2694.2017.00.005
- VernacularTitle:2014-2015年商洛市流感病毒H3N2亚型血凝素(HA1)基因进化研究
- Author:
Yan-Liang QI
1
;
Wei SHI
;
Yong-Bo DUAN
;
Ji-Ru XU
;
Kan-Ji DAI
;
Fang-Fang ZHANG
;
Peng-Bo YU
Author Information
1. 西安交通大学医学部
- Keywords:
influenza virus;
H3N2 subtype;
hemagglutinin;
vaccine strain;
genetic evolution
- From:
Chinese Journal of Zoonoses
2018;34(1):6-11
- CountryChina
- Language:Chinese
-
Abstract:
Comparative analysis of the variations in HA 1 gene of the influenza A (H3N2) virus and the vaccine recommended were conducted in Shangluo city of China,during the surveillance year of 2014-2015.In this study,we collected the samples of H3N2 subtype strain from the Shanglou City of China during the surveillance period of 2014-2015.The strain was cultured in MDCK cells,HA gene fragment was amplified by RT-PCR and the nucleotide sequence was determined.Sequence alignment was performed using the clustax2.1 software.The phylogenetic tree was constructed by Mega6.0 software and was analyzed by Neighboring-joining method.Results showed that the homology of isolated strain during 2014-2015 was 97.2 ％-99.9％ and homology with the recommended vaccine strain A/Texas/50/2012 was 97.3％-98.5％.The amino acid sequence of the HA 1 gene of the isolated strain was compared with that of the vaccine strain.The major antigenic determinants of the isolates in 2014,having mutations were section B,Y159F,S198P,while the major antigenic determinants of isolates in 2015,having amino acid mutations were A zone G142R,B region S159F,S198P.These results indicated that the key antigenic determinant of influenza H3N2 subtype strain in Shangluo City has changed in 2014-2015 and A/Texas/50/2012 vaccine component is no more effective.Hence,there is an urgent need to update the influenza H3N2 subtype vaccine components and in future we should be deeply concerned about the evolution ofinfluenza H3N2 gene trends.
