Complete genome sequence analysis of Hantaan virus strain AYW89-15 isolated in Jiangxi, China
10.3969/j.issn.1002-2694.2017.12.007
- VernacularTitle:江西省汉滩型汉坦病毒分离株AYW89-15全基因组序列分析
- Author:
Shi-Wen LIU
1
;
Tian GONG
;
Gang XU
;
Yong SHI
;
Jian-Xiong LI
;
Yan-Ni ZHANG
;
Jun ZHOU
;
Xiao-Qing LIU
;
Fang XIAO
;
Ying XIONG
Author Information
1. 江西省疾病预防控制中心
- Keywords:
Hantaan virus;
complete gene sequence;
genotype
- From:
Chinese Journal of Zoonoses
2017;33(12):1089-1093
- CountryChina
- Language:Chinese
-
Abstract:
We investigated the complete genome characteristic of the Hantaan virus strain AYW89-15 isolated in Jiangxi Province,China.Primers were designed and were used to amplify the complete S,M and L segments by RT-PCR.The PCR product were then cloned and sequenced,the gene sequences were analyzed with DNAStar and MEGA6.0 software.Result showed that the complete gene sequence was 11 848 nucleotides in length,the S,M and L segments were 1 699 nt,3 616 nt and 6 533 nt respectively,encoding 429,1 133 and 2 151 amino acids respectively.The sequence identities between stain AYW89-15 and other Hantaan virus were 79.7％-87.3％ at the nucleotide level and 92.3％-98.4％ at the amino acid level.Phylogenetic analysis of HV showed AYW89-15 belonged to a new HTNV lineage.AYW89-15 was a new subtype of HTNV that exists in Jiangxi Province.
