Helicobacter pylori Tipα protein induces IL-1β and IL-18 production in THP-1-drived macrophages through activation of NLRP3 inflammasome
10.3969/j.issn.1002-2694.2017.12.004
- VernacularTitle:幽门螺杆菌Tipα蛋白激活NLRP3炎性小体诱导THP-1细胞分泌IL-1β和IL-18
- Author:
Ling-Qiao XIAO
1
;
Chao WANG
;
Cheng-Yuan XIE
;
Shuo LIU
;
Guo-Dong CHEN
;
Jie LI
;
Min-Jun YU
;
Lan-Hua ZHAO
;
Liang HAN
;
Yan ZHANG
Author Information
1. 南华大学病原生物学研究所
- Keywords:
Helicobacter pylori;
Tipα;
NLRP3 inflammasome;
IL-1β;
IL-18
- From:
Chinese Journal of Zoonoses
2017;33(12):1071-1076
- CountryChina
- Language:Chinese
-
Abstract:
We preliminarily investigated the role of NLRP3 inflammasome in Helicobacter pylori (H.pylori) Tipa-induced pro-inflammatory cytokines secretion in PMA-differentiated human acute monocytic leukemia cell line THP-1.PMA-differentiated THP-1 cells were treated with pure recombinant Tips protein.The secretion levels of TNF-α,IL-1β and IL-18 in supernatant of culture medium were detected by ELISA.Then we blocked MyD88/NF-κB and NLRP3/Caspase-1 pathways by PDTC or the general ROS scavenger,NAC,respectively,and determined the secretion levels of proinflammatory cytokines and the expression levels of NLRP3 and Caspase-1.The results showed that Tips protein can significantly induced the secretion of TNF-α,IL-1β and IL-18 in THP-1 cells in a time and dose-dependent manner.Levels of TNF-α,IL-1β and IL-18 approached their peaks at 6 h post-treatment by 40 μg/mL of Tipα protein (P＜0.05).Moreover,the blockade of NF-κB signaling pathway by PDTC can inhibit the secretion of proinflammatory cytokines and the expression of NLRP3 and Caspase-1.When THP-1 cells were pre-treated with ROS scavenger NAC,the Tipα-induced increased IL-1β and IL-18 secretion was obviously eliminated (P＜0.05),while TNF-α level had no significant difference,and the expression levels of Caspase-1 and NLRP3 also have a significant decrease.Our results demonstrated that Tipα can promote THP-1-drived macrophages to secrete proinflammatory cytokines TNF-α,IL-1β and IL-18,and the NLRP3/Caspase-1 pathway may be involved in the Tipα protein-induced IL-1β and IL-18 secretion.
