Species identification of Triculinae based on COI gene segment, Fujian Province

VernacularTitle:基于COI基因片段的拟钉螺种类鉴定初探
Author: Xue-Mei KE ¹ ; Min QIU ; Chen-Xin LIN ; Jing GE ; Zhi-Nan GUO ; Ming-Ji CHENG ; Qing CHEN ; Guo-Wei CHEN
Author Information

1. 厦门市疾病预防控制中心
Keywords: Triclulinae; COI; species classification phylogenetic
From: Chinese Journal of Zoonoses 2017;33(8):724-729
CountryChina
Language:Chinese
Abstract: The aim of the study is to evaluate the utility value of species identification of Triculinae based on COI gene.Triculinae-like samples were collected from different places in Fujian Province.After classification based on morphology characters,genomic DNA was extracted from collected samples.Then the segments of COI gene were amplified and sequenced for taxonomy annotation and phylogenetic analysis.Eleven samples were annotated as Gammatricula (identities:88.96％-97.82％),the other two were annotated as Tricula wumingensis (identity:87.08％) and Neotricula apart (identity:88.55％),respectively.In most cases (12 out of 13),there was a difference between results based on different classification methods on a genus level.The alignment of COI gene segment is sufficient for preliminary identification of Triclulinae at family level.However,it is need further study in species identification of Triclulinae at genus level.