Detecting Salmonella with matrix assisted laser desorption ionization time of flight mass spectrometry
10.3969/j.issn.1002-2694.2017.08.008
- VernacularTitle:沙门菌基质辅助激光解吸电离飞行时间质谱方法研究
- Author:
Jian-Hong ZHAO
1
;
Yan GAO
;
Shu-Ming LI
;
Shi-Yao ZHANG
;
Heng-Wei WANG
;
Lin GU
;
Qing-Hua HAN
;
Yang JIAO
;
Qian CHEN
Author Information
1. 北京市朝阳区疾病预防控制中心
- Keywords:
matrix-assisted laser desorption ionization time of flight mass spectrometry MALDI-TOF-MS);
Salmonella;
identification
- From:
Chinese Journal of Zoonoses
2017;33(8):705-709
- CountryChina
- Language:Chinese
-
Abstract:
In order to optimize the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) method for Salmonella identification,study the target board pollution situation of different pretreatment methods,and establish a safe and effective method for detection of Salmonella,we applied MALDI-TOF-MS technique to detect 4 standard strains-of Salmonella under different culture conditions and sample preparation method,and established optimization program.MALDI target board was detected under different preparation treatments.The optimization method was used to detect 33 strains Salmonella which isolated from diarrhea patients' stool.Identification results were compared with serological results.The study found that MALDI-TOF-MS method could accurate identify of Salmonella in different pretreatment methods and culture medium.Thirty-three strains of Salmonella identified by MALDI-TOF-MS method were all accurate appraisal in genus level,19 strains of them were completely consistent with the serotyping identification results,14 strains of them were not consistent with the serotyping identification results.There was no bacteria growth on the target board in different pretreatment methods.MALDI-TOF-MS method can in a fast,convenient,safe and accurate way identify Salmonella,and it can become an effective means for identification of Salmonella.
