Laboratory investigation of a clustered respiratory tract infection with human adenovirus
10.3969/j.issn.1002-2694.2017.08.004
- VernacularTitle:一起腺病毒引起的聚集性呼吸道感染的实验室调查
- Author:
Ying ZHU
1
;
Qi LIN
;
Li-Bin YOU
;
Zhi-Miao HUANG
;
Min-Hong CHEN
;
Xu YAO
;
Yong-Jun ZHANG
;
Jian-Ming OU
;
Yu-Wei WENG
Author Information
1. 福建省疾病预防控制中心
- Keywords:
human adenovirus;
viral isolation;
sequence analysis
- From:
Chinese Journal of Zoonoses
2017;33(8):685-689
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the pathogens associated with a clustered event of fever occurred in a kindergarten in Fuzhou,Fujian Province,samples were collected from pediatric cases in the kindergarten and screened for various possible viral agents by real-time PCR.Of 10 respiratory specimens,7 were positive of human adenovirus (HAdV).The positive samples were inoculated into HEp-2 cell-lines for viral isolation.The PCR products of the hypervariable regions of Hexon gene were sequenced,followed by BLAST searches for viral type identification.In comparison with the strains prevalent in domestic or abroad in recent years,the deduced amino acid sequences showed no amino acid mutation in the hypervariable regions of Hexon.Combined with clinical manifestation and field epidemiological data,human adenovirus type 7 could be confirmed as the pathogen linked to the clustered event.
