Polymorphisms at HLA-A,-B,-DRB1 loci in Dalian Han population
10.3969/j.issn.1000-484X.2018.06.024
- VernacularTitle:大连地区汉族人群HLA-A,-B,-DRB位点基因多态性研究
- Author:
Lin-Nan SHAO
1
;
Shu-Ting ZHANG
;
Ying DUAN
;
Xiao-Hua LIANG
;
Wei-Jian YU
;
Shi-Hang ZHOU
Author Information
1. 大连市血液中心
- Keywords:
Dalian;
HLA;
Haplotype;
Linkage disequilibrium;
Genetic distance
- From:
Chinese Journal of Immunology
2018;34(6):919-924,928
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the polymorphisms at HLA-A,-B,-DRB1 loci in Dalian Han population. Methods: A total of 10 000 unrelated marrow donors who live in Dalian were genotyped by SBT and SSO methods. Haplotype frequencies and linkage dis-equilibrium values were calculated by ARLEQUIN software,and DA genetic distances between populations were calculated by poptree2 software. Results: A total of 18 HLA-A alleles, 32 HLA-B alleles and 13 HLA-DRB1 alleles were found in Dalian Han population. HLA-A?02 (31. 65% ),B?40(14. 84% ) and DRB1?15(15. 82% ) occurred most frequently. A?30-B?13-DRB1?07 (4. 56% ) was determined to be the most common three-locus haplotype and the second predominant haplotype was A?02-B?46-DRB1?09 ( 2. 43% ) . A ?30-B ?13 ( 6. 00% ) and B ?13-DRB1 ?07 ( 59. 89% ) were the most common two-locus haplotypes. Moreover,A?33-B?58 and B?13-DRB1?07 were strongest haplotypes with the linkage disequilibria values 0. 336 6 and 0. 665 1,respectively. In China,the closest genetic distances were found with Heilongjiang (0. 001) followed by Jilin (0. 002) and Shandong (0. 002),the furthest was found with Taiwan (0. 047). Compared with other populations worldwide,the closest genetic distances were found with Thailand (0. 029) and Korea (0. 03),the furthest was found with Italy (0. 183). Conclusion: Dalian Han population had rich polymorphism at HLA-A,-B,-DRB1 loci,and the distribution of HLA-A,-B and-DRB1 was in line with the charac-teristics of the northern population.
