Correlation research between JAK2-STAT3/STAT5 signal pathway and CD4+T lymphocyte subsets in children with food allergy
10.3969/j.issn.1000-484X.2018.06.019
- VernacularTitle:JAK2-STAT3/STAT5 信号通路与儿童食物过敏 CD4+T淋巴细胞亚群变化的相关性研究
- Author:
Xiao-Yan ZENG
1
;
Bo HU
;
Chuan-Ying LI
;
Bi-Quan CHEN
Author Information
1. 安徽医科大学第一附属医院儿科
- Keywords:
JAK2;
STAT3;
STAT5;
Signal pathway;
Children′s food allergy;
CD4+T lymphocyte
- From:
Chinese Journal of Immunology
2018;34(6):897-901
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the regulation of JAK2-STAT3/STAT5 signaling pathway on CD4+T lymphocyte subsets in children with food allergy. Methods:78 children with food allergy from January 2015 to March 2017 in our hospital were enrolled as the allergy group,and 78 healthy children in our hospital were enrolled as the control group. All the patients were divided into A,B,C,D groups according to ages. A group was less than or equal to 1 year old,B group of 1-3 years old(not including 1 year old),group C 3-6 years old(not including 3 years old),group D 6-11 years old(not including 6 years old). Flow cytometry was used to detect the CD4+T lymphocyte subsets Th1,Th2,Th17 and regulatory T cells(Treg) and qPCR was used to detect the mRNA expression of peripheral blood mononuclear cells of JAK2,STAT3,STAT5. Compare the CD4+T lymphocyte subsets and JAK2,STAT3,STAT5 expression level differences among two groups children and further analysis the correlation of JAK2-STAT3/STAT5 signal transduction pathway and CD4+T lymphocyte subsets. Results:The percentage of Th1 and Treg lymphocytes and Th1/Th2 and Treg/Th17 ratio of the group A,B, C in control group were significantly higher than the allergy group,while Th2,Th17 lower than allergy group. The expression mRNA level of JAK2,STAT3 and STAT5 in the control group were significantly lower than the allergic group,the difference was statistically significant(P＜0. 05). The correlation for JAK2,STAT3,STAT5 and CD4+T level of lymphocyte subsets were compared,correlation coefficient of Th1,Treg,Th1/Th2,Treg/Th17 were all higher than 0(P＜0. 05),and the correlation coefficient of Th2 and Th17 were smaller than 0(P＜0. 05). However in group D,there was no significance. Conclusion:The JAK2-STAT3/STAT5 signaling pathway in young children with food allergy has obvious activation and CD4+T lymphocyte subsets change,and the JAK2-STAT3/STAT5 signaling pathway can effectively regulate the distribution of CD4+T lymphocyte subsets. With the age of children increasing,the correlation of these effects is weakened and disappeared.
