Preparation of immune microarray carrier based on agarose self-assembled membrane and its application in probe immobilization
10.3969/j.issn.1000-484X.2018.06.014
- VernacularTitle:琼脂糖自组装膜基底型免疫芯片载体制备及抗体固定研究
- Author:
Li-Ping MA
1
;
Sheng-Long MA
;
Yun-Xia LI
;
Ying-Ying NIE
;
Gen-Liang HAN
Author Information
1. 甘肃省科学院传感技术研究所
- Keywords:
Agarose nano-membrane carrier;
Immune microarray;
Antibody immobilization;
Fluorescence intensity
- From:
Chinese Journal of Immunology
2018;34(6):872-876
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To improve the immobilization efficiency of antibody molecules on immune microarray,the method of es-tablishment and optimization of agarose self-assembled membrane carrier with three-dimensional hydrogel structure was established. Methods: The agarose self-assembled membrane carrier was prepared by using glass slide as the carrier,using agarose and sodium periodate modification on glass surface. The agarose self-assembled membrane carrier was characterized by TEM, AFM and FTIR. The optimum preparation conditions were obtained. The carrier for two different species of fixed source antibody efficiency were studied. Antibody loading capacity of agarose self-assembled membrane carrier and ordinary aldehyde carrier were investigated and compared by fluorescence microscopy imaging and Image J software. Results: The agarose nano-membrane carrier had uniform and compact surface. This structure could increase the specific surface area and improve the probe fixed rate. The optimal concentration of agarose for preparation of carrier was 1. 0% . When the concentration of IgG was 0. 3-0. 4 mg/ml,the oxidized self-assembled chitosan film substrate had highest antibody loading capacity. And it had a 3. 94 fold higher antibody loading capacity than the ordinary aldehyde carrier. Conclusion: The agarose nano-membrane carrier is an ideal method for surface modification of immobilized antibody molecules, which is more suitable for preparation of immune microarray carrier.
