Proliferation inhibition effects of curcumin against CD44+colon cancer cells in vitro

VernacularTitle:姜黄素抑制CD44+结肠癌细胞增殖的体外实验研究
Author: Xue-Yan XI ¹ ; Fei FU ; Xu FAN ; Bo-Yu DU
Author Information

1. 湖北医药学院基础医学院
Keywords: Curcumin; Colon cancer; Cancer stem cells; CD44+
From: Chinese Journal of Immunology 2018;34(6):850-853
CountryChina
Language:Chinese
Abstract: Objective:To study confirm whether curcumin could inhibit the proliferation of CD44+colon cancer cells. In this study. Methods: MTT method was used to test the proliferation inhibition effects of curcumin against colon cancer cells. And Real-time PCR and Fluorescence-activated cell sorting (FACS) were used to determine CD44 expression level in colon cancer cells after treated with curcumin. Then we used Magnetic-activated cell sorting ( MACS) method to separate CD44 positive and negative cells. And we compared the proliferation inhibition effects of curcumin against these two kinds of cells. Results: Curcumin could inhibit the proliferation of HCT116 and HT-29 colon cancer cell lines. The expression level of CD44 could also be down-regulated after the colon cancer cells were treated with curcumin. Conclusion: Curcumin could inhibit the proliferation of CD44+colon cancer cells. Thus it was suggested that crcumin might have the effect to inhibit the proliferation of colon cancer stem cells and might be used as a promising agents to prevent the relapse and metastasis of colon cancer.