Duplex effect of CCK8 and PGE2 in CVB-attacked human peripheral blood pDC
10.3969/j.issn.1000-484X.2018.06.004
- VernacularTitle:CCK8和PGE2对CVB体外攻击人外周血pDC的调控
- Author:
Miao-Miao JIA
1
;
Li-Li QI
;
Hui LI
;
Xiang GAO
;
Wen-Xuan LIU
;
Xian-Xian JIA
Author Information
1. 河北医科大学基础医学院病原生物学教研室
- Keywords:
Plasmacytoid dendritic cells;
Coxsackievirus B;
CCK8;
PGE2
- From:
Chinese Journal of Immunology
2018;34(6):820-825
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the immunomodulation of CCK8 on the Coxsackievirus B ( CVB )-attacked human peripheral blood plasmacytoid dendritic cells(pDC). Methods:Peripheral blood mononuclear cells of healthy volunteers were separated by Ficoll-Hypaque gradient density centrifugation. The pDC was separated and divided into five groups,which were the control group, CVB attacked group,the group of CCK8 treated after CVB attack,the group of PGE2 treated after CVB attack and the group of CCK8+PGE2 treated after CVB attack. 100-time TCID50 of CVB was applied for the attack on pDC. Real-time PCR and Immunofluorescence technique were employed to detect the expression of CCK1R/CCK2R mRNA and protein. Then,the expression levels of costimulatory molecules such as CD80,CD86,HLA-DR ligand,and the chemokine receptor CCR7 were evaluated by Flow Cytometry Analysis. The supernatants of pDCs were collected, and the content of IFN-α was determined by Enzyme-linked Immunosorbent Assay. Results:CCK1R and CCK2R were co-expressed in human peripheral blood pDC,and both were significantly upregulated after CVB attack in vitro. Expression of CD80,CD86,HLA-DR and IFN-α were decreased in the CVB+CCK8 group compared with the CVB group,which suggested that CCK8 may reduce the CVB activation of pDC. Whereas expression of CD80,CD86,HLA-DR,CCR7 and IFN-α were increased in the CVB+PGE2 group compared with the CVB group,which suggested that PGE2 may increase the CVB activation of pDC in vitro. Conclusion:CCK8 repressed the CVB-attacked pDC,while PGE2 activated the CVB-attacked pDC.
