Interactions between induced T regulatory cells and B cells in inflammatory milieu in mice with collagen-induced arthritis
10.3969/j.issn.1000-484X.2018.06.003
- VernacularTitle:胶原诱导性关节炎炎症条件下诱导的调节性 T 细胞与 B 细胞的相互作用
- Author:
Ning KONG
1
;
Juan SUN
;
Yi-Ming YANG
;
He-Jian ZOU
;
Jie YANG
;
Wei-Guo WAN
Author Information
1. 复旦大学附属华山医院风湿科
- Keywords:
Rheumatoid arthritis;
Collagen-induced arthritis;
Induced T regulatory cells;
B cells;
Co-stimulator
- From:
Chinese Journal of Immunology
2018;34(6):815-819,825
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the interactions between induced T regulatory cells ( iTregs ) and B cells in the inflammatory milieu in mice with collagen-induced arthritis(CIA). Methods: CD19+ cells were isolated from the spleen cells of normal DBA1/J(N-B) mice and CIA mice(CIA-B) on the 35th day after the first immunization with established arthritis. These B cells were used as antigen-presenting cells to observe their effects on the induction of Tregs. Tregs were induced with the classic method and co-cultured with CIA-B cells. CIA-B cell effects on iTreg proliferation and the expression of CTLA-4 on iTregs were explored. iTregs′ influence on the expressions of co-stimulators(CD80,CD86) and MHCⅡon B cells was studied and its mechanism was determined by the Transwell experiments. Results: CIA-B could induce more Treg production and proliferation. CIA-B could also promote the CTLA-4 expression on iTreg cell surface which worked through a cell-contact pathway, while iTregs could increase the expressions of co-stimulators(CD80,CD86) and MHCⅡby the same way. Conclusion: iTregs could show their immune suppressive function through the interactions with CIA-B cells in the inflammatory milieu in mice with CIA. These interactions work by a cell-contact pathway.
