Overexpression of TLR2,TLR4 and MyD88 is associated with inflammation in C3H mice during Chlamydia respiratory infection
10.3969/j.issn.1000-484X.2018.05.019
- VernacularTitle:TLR2、TLR4及MyD88高表达与Cm呼吸道感染肺组织炎症相关
- Author:
Gao-Ju PANG
1
;
Li-Da SUN
;
Nan YAO
;
Xiao-Yu ZHA
;
Ju-You LIANG
;
Lu TAN
;
Hong ZHANG
;
Sai QIAO
;
Hong BAI
Author Information
1. 天津医科大学基础医学院免疫学系
- Keywords:
Chlamydia muridarum;
C3H mouse;
TLR2;
TLR4;
MyD88
- From:
Chinese Journal of Immunology
2018;34(5):737-740
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of excessive inflammation in the lung of C3H/HeN(C3H) mice following Chlamydia muridarum(Cm) airway infection.Methods:Chlamydial pneumonitis was induced in C3H and C57BL/6(C57) mice by intranasal inoculation with 1×103IFU (inclusion forming unites) of Cm strains.The expression of TLR2,TLR4 and MyD88 mRNA in the lung at different time point post-infection was measured by RT-PCR.Results:Cm infection induced Toll-like receptors expression in two strains of mice.The expression of TLR2 and TLR4 mRNA,especially TLR2 mRNA(P<0.001 or P<0.05),were significantly higher in highly susceptible C3H mice on day 7 and day 14 d post-infection compared with C57 mice.Further studies showed that the expression of MyD88 mRNA was also significantly higher in C3H mice on day 7 post-infection,and maintained high expression untill the day 14.Conclusion:Cm lung infection induced high level of TLR2,TLR4 and MyD88 mRNA expression in C3H mice,which may associate with excessive inflammation in C3H mice.
