Screening and identification of human monoclonal antibodies to hepatitis E virus capsid protein
10.3969/j.issn.1000-484X.2018.05.012
- VernacularTitle:戊型肝炎病毒衣壳蛋白特异性人源单克隆抗体的筛选与鉴定
- Author:
Xu ZHANG
1
;
Gui-Ping WEN
;
Zi-Min TANG
;
Si-Ling WANG
;
Jia-Xin CHEN
;
Dong YING
;
Chang LIU
;
Zi-Zheng ZHENG
;
Ning-Shao XIA
Author Information
1. 厦门大学分子疫苗学与分子诊断学国家重点实验室
- Keywords:
Hepatitis E virus;
Capsid;
Human monoclonal antibody;
Flow cell sorting;
Single cell RT-PCR
- From:
Chinese Journal of Immunology
2018;34(5):703-707
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for rapidly screening human antibodies recognizing HEV capsids proteins from pe-ripheral blood.The antibodies recognizing HEV capsids proteins were screened from the peripheral blood of vaccinator and the properties of the antibodies were analyzed.Methods:The HEV capsids proteins specific memory B cells in peripheral blood were obtained by flow cytometry sorting.Then antibody variable genes were acquired through single-cell RT-PCR and recombined to express in eukaryocyte.Finally,the properties analysis of recombinant expressed human monoclonal antibodies were carried out.Results:Six hu-manized monoclonal antibodies recognizing HEV capsids proteins were successfully obtained,and most of them had binding activity and neutralizing activity.Conclusion:The sequence of human monoclonal antibodies recognizing HEV capsid proteins is successfully screened and successfully expressed in the eukaryocyte.The properties of the antibodies are identified,which lay the foundation for studying antibody evolution in the human body after vaccination.
