Regulatory effects and mechanism of aconitine on proliferation,invasion and migration of hepatoma carcinoma cell MHCC97
10.3969/j.issn.1000-484X.2018.05.009
- VernacularTitle:乌头碱对肝癌MHCC97细胞生长、侵袭和迁移的调控作用及机制研究
- Author:
Hui-Sheng XIONG
1
;
Can JIANG
;
Rui GAO
;
Li-Hua CHEN
Author Information
1. 重庆市肿瘤研究所中医肿瘤科
- Keywords:
Aconitine;
Hepatoma carcinoma cell;
Proliferation;
Invasion;
Migration
- From:
Chinese Journal of Immunology
2018;34(5):688-692
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the anticancer activity and mechanism of aconitine on cell proliferation,invasion and migration of hepatoma carcinoma cell(HCC).Methods:The effect of aconitine at different concentrations on proliferation was calculated by MTT assay.The effects of aconitine on invasion and migration of HCC were measured by Transwell and wound healing assay.Western blot was employed to detect the protein levels of P38MAPK signaling pathway-related proteins.Results:The concentrations of 5,10,20 μg/ml were selected according to the results of pre-experiment.Aconitine(10,20 μg/ml) inhibits proliferation and invasion of MHCC97 cells markedly after cells were treated with aconitine for 4 days.Treatment with aconitine down-regulated the ability of migration and decreased the ratio of p-P38/P38 and protein levels of p-MAPKAPK and p-HSP27.Conclusion:Aconitine inhibits prolif-eration,invasion and migration,and the mechanism may related with P38MAPK signaling pathway.
