Study on effects and mechanisms of long non-coding RNA UCA1 on cell survival and migration of bladder cell UM-UC-3 via targeting miR-582-5p
10.3969/j.issn.1000-484X.2018.05.006
- VernacularTitle:长链非编码RNA UCA1靶向miR-582-5p对膀胱癌UM-UC-3细胞生存和运动能力的调节作用及机制研究
- Author:
Zhe WANG
1
;
Jing ZHANG
;
Huai-An CHEN
;
Chao ZHANG
;
Shuo LIU
;
Wen-Long MIAO
Author Information
1. 河北北方学院附属第一医院泌尿外科
- Keywords:
UCA1;
MiR-582-5p;
Bladder cancer;
Proliferation;
Apoptosis;
Migration;
Invasion
- From:
Chinese Journal of Immunology
2018;34(5):670-674,680
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects and mechanisms of long non-coding RNA UCA1 on cell survival and migration of bladder cancer cell UM-UC-3 by targeting miR-582-5p.Methods:Cells were transferred with UCA1 shRNA(sh-UCA1)and(or)miR-582-5p,the transfection efficiency and level of miR-582-5p were detected by RT-PCR.The luciferase report assay was performed for validate the relationship of UCA1 and miR-582-5p.The cell viability was measured by CCK8 assay.Apoptosis was detected by flow cy-tometry.The metastatic ability was calculated by wound healing and Transwell assay.And the protein levels of proliferation-,apoptosis-and migration-related were determined by Western blot.Results:sh-UCA1 inhibited the expression of UCA1 and induced the expression of miR-582-5p(P<0.05),and miR-582-5p inhibitor alleviated the effect of UCA1 on miR-582-5p(P<0.05).The luciferase reporter assay indicated that there was miR-582-5p binding site on UCA1.Silencing of UCA1 inhibited proliferation of bladder cancer cells and the expression of Ki67,induced apoptosis and expression of cleaved caspase-3(P<0.05).Meanwhile,sh-UCA1 inhibited migration and invasion of bladder cancer cells coupled with decreasing VEGF(P<0.05).In addition,miR-582-5p inhibitor attenuated the effects of UCA1 on proliferation,apoptosis and migration(P<0.05).Conclusion:UCA1 promotes survival and migration of bladder cancer cells through targeting miR-582-5p.
