Effects of CXCL4 on human dermal microvascular endothelial cells and secretion of vasomotion factors
10.3969/j.issn.1000-484X.2018.05.005
- VernacularTitle:CXCL4对人真皮微血管内皮细胞的功能及分泌血管舒缩因子的影响
- Author:
Zhi-Xing JIANG
1
;
Sen YANG
;
Chen CHEN
;
Min-Rui LIANG
;
He-Jian ZOU
Author Information
1. 复旦大学附属华山医院风湿科
- Keywords:
CXCL4;
Systemic sclerosis;
Endothelin-1;
Fli-1;
AT1R
- From:
Chinese Journal of Immunology
2018;34(5):665-669
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify the proliferation effect and angiogenic ability of CXCL4 on human dermal microvascular endothelial cells(HDMECs),and to explore the secretion of vasomotion factors.Methods:HDMECs were treated with gradient concentration to test the proliferation of HDMECs.CCK-8 was used to explicated the proliferation of HDMECs.The effect of CXCL4 on angiogenic ability of HDMECs was determined by tube formation assay.The mRNA levels of endothelin-1(ET-1),Fli-1,AngiotensinⅡtype 1 receptor(AT1R) and endothelin-1 type A receptor (ETAR) were detected by real-time quantitative polymerase chain reaction (Real-time PCR).Results:The specific receptor of CXCL4 was expressed on HDMECs.CXCL4 could inhibit the proliferation of HDMECs and the number of tube formation in a dose-depend manner.After CXCL4 intervention,the relative amplification multiples of ET-1,AT1R were significantly increased(P<0.05),Fli-1 was decreased(P<0.05),and ETAR had no change as compared with the control group.Furthermore,CXCL4 antagonist could reverse the effects of CXCL4 on HDMECs.Conclusion:CXCL4 inhibit the proliferation and angiogenesis of HDMECs and induce the secretion of ET-1 and AT1R,reduce the secretion of Fli-1 in a dose-dependent manner.
