Effects of miR-34 on migration and invasion of prostate cancer cells by MSR1 protein
10.3969/j.issn.1000-484X.2018.04.013
- VernacularTitle:miR-34a调控MSR1蛋白对前列腺癌细胞迁移和侵袭的影响
- Author:
Xing-Tao HAN
1
;
Ling-Bo YANG
;
Peng-Tao WEI
;
Xiao-Hui LI
;
Jian-Tao SUN
;
Jin-Jian YANG
Author Information
1. 洛阳市中心医院泌尿外科
- Keywords:
miR-34a;
Prostate cancer;
MSR1;
Invasive ability
- From:
Chinese Journal of Immunology
2018;34(4):544-548
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of miR-34a on the migration and invasion of prostatic cell line through MSR1.Methods:Western blot was used to detect the expression of MSR1 in prostate cancer cell lines.qPCR was used to detect the ex-pression of miR-34a in prostate cancer cell lines.The correlation between miR-34a and MSR1 was examined by double luciferase as-say.Transwell invasion assay was used to detect the effect of miR-34a and MSR1 on the invasion ability of prostate cancer cells.The effects of miR-34a and MSR1 on the fine migration ability of prostate cancer were examined by scratch healing.Results:The expression level of MSR1 in PC3 cells was relatively high by Western blot.The expression level of miR-34a in PC3 cells was relatively low by qPCR.Double luciferase assay showed a direct binding site between miR-34a and MSR1,MiR-34a could regulate the expression level of MSR1,Scaffold Healing Test and Transwell Invasion Test were used to detect the migration and invasion of PC3 cells after overexpressing miR-34a.Overexpression of MSR1 could reverse the inhibitory effect of miR-34a on the migration and invasion of PC3 cells.Conclusion:miR-34a can regulate the migration and invasion of prostate cancer cells through MSR1 protein.
