Effects of short-term treatment of dexamethasone on balance of natural regulatory and type Ⅰ regulatory T cells
10.3969/j.issn.1000-484X.2018.04.003
- VernacularTitle:地塞米松体外短期处理影响Ⅰ型调节性T细胞与自然调节性T细胞平衡
- Author:
Li-Yan HUANG
1
;
Zhi-Bing OU
;
Ting XIE
;
Xiang-Ping LIAO
;
Jian ZHANG
;
Wen-Pei LIU
;
Xiao-Wang QU
Author Information
1. 南华大学附属郴州医院转化医学研究所
- Keywords:
Glucocorticoid;
Dexamethasone;
Natural regulatory T cell;
Type Ⅰ regulatory T cell
- From:
Chinese Journal of Immunology
2018;34(4):492-495,501
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the impact of dexamethasone on the balance of natural regulatory T cells(nTreg) and typeⅠ regulatory T cell(Tr1) in vitro.Methods:Peripheral blood lymphocytes(PBMCs) recruited from healthy donor,divided into dexam-ethasone stimulus group and negative control group.After 3 days treatment,cells were stained.The expression of CD25,CD127, Lymphocyte-activation 3 (LAG-3) and Forkhead box P3 (Foxp3) and frequency of Treg and Tr1 were analyzed by flow cytometry.Results:Compared to the control group,after stimulation with dexamethasone for 3 days,the percentage of CD4+T cells increased in a dose-dependent manner.The expression of CD25 and Foxp3 in CD4+T cells decreased significantly (P=0.006, P<0.000 1),while CD127 and LAG-3 expression increased significantly in CD4+T cells (P<0.000 1,P=0.011).Dexamethasone treatment significantly enhance the frequency of Tr1(P=0.051),reduce the frequency of Treg (P<0.001),and the ratio of Tr1/Treg also increased(P=0.044).Conclusion:Short-term treatment with dexamethasone in vitro change the balance of natural regulatory and type Ⅰ regulatory T cells.
