Effect of down-regulation of miR-21 on proliferation and apoptosis of leukemic cells by inhibiting PI3K/AKT signaling pathway
10.3969/j.issn.1000-484X.2018.03.025
- VernacularTitle:下调miR-21抑制PI3K/AKT信号通路对白血病细胞增殖凋亡的影响
- Author:
Kuang-Hua YAN
1
;
Long CHEN
;
Feng-Meng YAN
Author Information
1. 河南大学第一附属医院血液科
- Keywords:
miR-21;
Leukemia;
Cell proliferation;
Apoptosis;
PI3K/AKT signaling pathway
- From:
Chinese Journal of Immunology
2018;34(3):441-445
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of miR-21 on the proliferation and apoptosis of leukemia cell line K562 and the regulation of PI3K/AKT signaling pathway.Methods:K562 cells were divided into control group,miR-21 NC group and miR-21 interference group,the first group without treatment,the two groups after using cationic liposome transfection of LipofectamineTM2000 miR-21 inhibitor and miR-21 negative control.After transfection 48 h,the expression of miR-21 mRNA in cells was detected by Real-time fluorescence quantitative (qRT-PCR),MTT assay was used to test the effect of miR-21 on cell proliferation,flow cytometry was used to detected the effect of miR-21 on cell cycle apoptosis rate,the protein expression of PI3K,AKT and p-AKT in PI3K/AKT signaling pathway after miR-21 transfection were detected by protein immunoblotting (Western blot).Results:The expression level of miR-21 mRNA and the survival rate of cells in the miR-21 interference group were significantly lower than those in the control group and the miR-21 NC group;compared with control group and miR-21 group NC,flow cytometry detected the cells accounted for proportion of G0/G1 phase in miR-21 interference group increased significantly,the proportion of cells at S phase significantly decreased,and apoptosis rate increased significantly.Western blot test results show that the expression levels of p-AKT in miR-21 inter-ference group were significantly lower than those in the control group and miR-21 NC group,but the expression levels of PI3K and AKT protein were not changed significantly.Conclusion:Down-regulation of miR-21 can inhibit the proliferation and promote apoptosis of leukemia K562 cells,and its mechanism may be related to the inhibition of PI3K/AKT signaling pathway.
