Effect of astragalus polysaccharides on apoptosis,transdifferentiation and ROS content in renal tubular epithelial cells of diabetic nephropathy
10.3969/j.issn.1000-484X.2018.03.014
- VernacularTitle:黄芪多糖对糖尿病肾病肾小管上皮细胞凋亡、转分化及ROS含量的影响研究
- Author:
Xiao-Ling GUO
1
;
Li-Xia KANG
;
Mei-Fang REN
;
Jin-Chuan TAN
Author Information
1. 河北省中医院肾病科
- Keywords:
Astragalus polysaccharides;
Diabetic nephropathy;
Renal tubular epithelial cells
- From:
Chinese Journal of Immunology
2018;34(3):388-392
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Astragalus polysaccharides on apoptosis,transdifferentiation and ROS content in renal tubular epithelial cells of diabetic nephropathy.Methods:HK-2 cells were divided into low glucose group,high glucose group and astragalus polysaccharide+high glucose group,cell proliferation was detected by CCK-8 assay after 48 h;cell apoptosis and ROS content was detected by flow cytometry;the expression of E-cadherin,α-SMA,STAT1,STAT3,p-STAT1,p-STAT3 protein were detected by Western blot.Results:The survival rate of cells in high glucose group was significantly lower than low sugar group (P<0.01),cell apoptosis rate,ROS content and E-cadherin,α-SMA,p-STAT1 and p-STAT3 protein expression was significantly higher than low sugar group (P<0.01),the cell survival rate in high glucose+astragalus polysaccharide group was significantly higher than high glucose group,cell apoptosis rate,ROS content and E-cadherin,α-SMA,p-STAT1 and p-STAT3 protein expression was significantly lower than high glucose group (P<0.01).Conclusion:Astragalus polysaccharides can promote the proliferation of renal tubular epithelial cells induced by high glucose,inhibit apoptosis and transdifferentiation,and the mechanism is related to down regulation of JAK/STAT signaling pathway.
