Mechanism of RNA interference HOXB7 gene expression in colon cancer on pro-liferation and apoptosis of cancer cells
10.3969/j.issn.1000-484X.2018.03.011
- VernacularTitle:RNAi结肠癌HOXB7基因表达对癌细胞增殖及凋亡的机制研究
- Author:
Ke-Xiang ZHU
1
;
De-Feng YUAN
;
Zheng-Cong ZHANG
;
Peng-Fei LÜ
;
Xiao-Ping BAI
Author Information
1. 兰州大学第一医院普外二科
- Keywords:
RNAi;
Colon cancer;
HOXB7 gene;
Notch1 signaling pathway
- From:
Chinese Journal of Immunology
2018;34(3):371-375
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of inhibiting of HOXB7 gene expression on proliferation and apoptosis of colon cancer cells.Methods:The synthetic negative control siRNA (negative control group) and HOXB7-siRNA (HOXB7 transfection group) were transfected into human colon cancer SW480 cells by LipofectamineTM2000 liposome mediated method,untreated cells as blank group.RT-PCR and Western blot were used to detect the mRNA and protein expression of HOXB7 after transfected 48 h respectively;cell proliferation was detected by CCK8 assay after transfected 24,48,72,96 h;cells apoptosis was detected by flow cytometry after transfected 48 h;the expression of apoptosis related proteins Bcl-2,Bax and Notch1 signaling pathway Notch1 and Hes1 were detected by Western blot.Results:The mRNA and protein expression of HOXB7 in HOXB7 transfected group was significantly lower than that in blank group(P<0.05);OD value was no statistical significance in the three groups after transfected 24 h(P>0.05), while after transfected 48,72,96 h,compared with the control group,OD value in HOXB7 group was significantly decreased(P<0.05);compared with the blank group,the apoptosis rate in HOXB7 transfection group increased significantly,and the expression of Bcl-2, Notch1 and Hes 1 proteins was down regulated,and the expression of Bax protein was up-regulated(P<0.05).Conclusion:RNA inter-ference in the expression of HOXB7 gene in colon cancer can inhibit the proliferation of cancer cells and induce apoptosis by inhibiting of the Notch1 signaling pathway.
