Experimental study of anti-metastasis effects of HUVEC vaccine combined with chemotherapy in EMT-6 breast cancer
10.3969/j.issn.1000-484X.2018.03.010
- VernacularTitle:HUVEC疫苗联合化疗抗EMT-6乳腺癌转移实验研究
- Author:
Mei-Yu LU
1
;
Wei-Lan ZHONG
;
Wan-Fa DONG
;
Qing-Shou YAO
;
Chun-Feng SI
;
Ling ZHOU
;
Mao-Lei XU
Author Information
1. 滨州医学院药学院 "方剂效应与临床评价"国家中医药管理局重点实验室
- Keywords:
Human umbilical vein endothelial cells;
Anti-angiogenesis;
Breast cancer;
Combination therapy;
Paclitaxel
- From:
Chinese Journal of Immunology
2018;34(3):367-370,375
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether human umbilical vein endothelial cell(HUVEC) vaccine combined with low dose docetaxel (DOC) could play a synergistic role in anti-breast cancer.Methods:BALB/c mice were randomly divided into normal saline group,HUVEC vaccine group,DOC group,and HUVEC vaccine combined with DOC treatment group (HUVEC-DOC) group.An experimental metastasis model by tail vein injection of EMT-6 breast cancer cells was employed to evaluate the anti-metastatic efficiency of the HUVEC-DOC combination treatment regime.Lymphocyte proliferation assay,cytotoxic T lymphocytes and an indirect enzyme-linked immunosorbent assay (ELISA) for detecting IFN-γ were used to investigate cellular immune responses elicited by the combination treatment regime.Results:Compared with HUVEC and DOC single drug group,the number of lung metastasis in HUVEC-DOC combination treatment group was significantly decreased(P<0.05).In vitro analysis of splenocytes isolated from immunized mice revealed an induction of cytotoxic T lymphocytes(CTLs) with a lytic activity against activated endothelium.IFN-γ in the serum of im-munized mice of the HUVEC-DOC combination treatment group was significantly higher than that in the other three groups(P<0.05). Conclusion:HUVEC vaccine with low dose of DOC could display synergistic anti-breast cancer effect.
