Effects of inhibiting of BAG-1 gene expression by RNA interference on prolifer-ation and apoptosis of cutaneous squamous cell carcinoma
10.3969/j.issn.1000-484X.2018.03.009
- VernacularTitle:RNA干扰抑制BAG-1基因表达对皮肤鳞状细胞癌增殖及凋亡的影响研究
- Author:
Feng XING
1
;
Si-Yu TAO
;
Li ZHANG
Author Information
1. 河南省人民医院眼科/河南省立眼科医院
- Keywords:
BAG-1 gene;
Cutaneous squamous cell carcinoma;
Apoptosis;
Signal pathway
- From:
Chinese Journal of Immunology
2018;34(3):362-366
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of inhibiting of BAG-1 gene expression on proliferation and apoptosis of cutaneous squamous cell carcinoma.Methods:siRNA of BAG-1 was transfected in cutaneous squamous cell carcinoma A431 by Lipo-fectamineTM2000,cells were transfected for 48 h,the BAG-1 mRNA expression was detected by RT-PCR,BAG-1 protein expression was detected by Western blot;CCK8 and flow cytometry were used to detect the cell proliferation and apoptosis;Western blot was used to detect the expression of Bax,β-catenin and Survivin protein;IL-6 and VEGF content were detected by ELISA kit.Results:Compared with control group and negative control group,transfection of BAG-1 with siRNA could significantly inhibit the expression of BAG-1 at the transcriptional and translational levels;compared with the control group,the cells proliferation in BAG-1-siRNA group significantly decreased,apoptosis rate was significantly increased,Bax protein expression was up-regulated,expression of β-catenin,Survivin protein were down regulated,the contents of IL-6 and VEGF were decreased significantly(P<0.05).Conclusion:Silencing of BAG-1 expression can reduce the proliferation of cutaneous squamous cell carcinoma,promote apoptosis,up regulate Bax expression and down regulate the expression of Wnt/β-catenin signaling pathway,and reduce the secretion of IL-6 and VEGF factors.
