Effect of hypobaric hypoxia on killing functions and secreting IL-6,TNF-α of peritoneal macrophages in mice
10.3969/j.issn.1000-484X.2018.03.006
- VernacularTitle:高原低氧环境对小鼠巨噬细胞杀伤活性及分泌IL-6、TNF-α的影响
- Author:
Xiao-Yan PU
1
;
Fang-Jie HU
;
Ji-Dong LI
;
Yan LIU
;
Li-Juan QIAO
;
Yu-Zhen XU
;
Jun LIU
;
Sheng YONG
Author Information
1. 青海大学医学院,西宁810001
- Keywords:
Hypoxia;
Macrophages;
Phagocytosis;
Respiratory burst;
NO
- From:
Chinese Journal of Immunology
2018;34(3):344-348
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the influence of high altitude hypoxia on the phagocytosis and killing functions of peritoneal macrophages in mice by establishing mouse model in high altitude hypoxic environment.Methods:①After exposure of mice to an altitude of 4 200 m,2 200 m and 400 m for 30 d respectively,flow cytometry was used to detect the phagocytosis and killing functions of peritoneal macrophages on staphylococcus aureus labeled with FITC.②The respiratory burst level of the cultured macrophage in mice was detected in 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescent probe method. ③The concentration of NO2- as a stable oxidative metabolite of NO in the supernatant of the cultured macrophages was measured with ELISA kits;④The release level of IL-6 and TNF-α in the cultured mice macrophage supernatant was also determined with ELISA kits.Results:After exposed under high altitude hypoxia for 30 d,compared with the control group (400 m),the phagocytosis,respiratory burst level and NO release in high altitude groups (4 200 m and 2 200 m) were all than those in the control group(400 m) (P<0.05).While the concentration of IL-6 and TNF-α in the Mφ cultured supernatant showed an obvious increase (P<0.05).Conclusion:Exposure under high altitude hypoxia (at altitude of 4 200 m and 2 200 m) for 30 d compromised the phagocytosis and oxygen dependent cytolyticactivity functions,and also raised the cytokines secretion level of IL-6 and TNF-α in Mφ,thereby affecting the innate immune response ability of Mφ in body.
