Effects of FoxO1 on FoxO1-KLF2-S1P1 pathway in Jurkat cells
10.3969/j.issn.1000-484X.2018.03.004
- VernacularTitle:FoxO1基因对Jurkat细胞FoxO1-KLF2-S1P1通路的调节作用
- Author:
Fei YAN
1
;
Ping-Ping LIU
;
Jian ZHANG
;
Rui ZHAO
;
Wen-Peng NI
;
Qian-Ru LI
;
Ying DU
Author Information
1. 郑州大学基础医学院免疫学系
- Keywords:
FoxO1;
Expression vector;
Interference vector;
S1P1;
CD62L
- From:
Chinese Journal of Immunology
2018;34(3):335-339
- CountryChina
- Language:Chinese
-
Abstract:
To observe the contribution of FoxO1 on its downstream molecules expression and function in Jurkat cells,Jurkat cells were infected with FoxO1 expression lentivirus and interference lentivirus to establish FoxO1-KLF2-S1P1 signaling pathways mod-el.After infection of 120 h,FoxO1,KLF2,S1P1 and CD62L mRNA levels and the protein expression of FoxO1,FoxO1-p and KLF2 were increased (P<0.05) in FoxO1-overexpression group.Converse results(P<0.05) were observed in the interference group.The proportions of S1P1+cells were increased in both groups.It was notably that S1P1+cells were decreased(P<0.05) in interference group after infection of 72 h.The proportion of CD62L+cells was increased(P<0.05) in overexpression group,it was decreased(P<0.05) in the interference group.This vitro experiments showed S1P1 and CD62L could be regulated by FoxO1 lentivirus,and provided an experimental basis for research about intracellular FoxO1-KLF2-S1P1 signaling pathways and cellular functions.
